Generator

Part:BBa_K539672:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

promoter (lacI regulated)+RBS+Alss with a RBS (B0032)+ilvC+a RBS (B0032)+ilvD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5194
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2562
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2148
    Illegal BsaI site found at 4980
    Illegal BsaI site found at 5237
    Illegal BsaI.rc site found at 535
    Illegal BsaI.rc site found at 1129
    Illegal BsaI.rc site found at 2967


Design Notes

Built in Plac, three weak expressing RBSs, Alss, ilvC and ilvD (Part:BBa_K539631 with Part:BBa_K539641).All of those sequences(Alss ,ilvC and ilvD) had been mutated to delet the function of RE site at the origin so that we can submit this part to the iGEM.


Source

Both of the ilvC and ilvD come from the Escherichia coli str. K-12 substr. MG1655. However,the Alss is from Bacillus subtilis.


References